*Masanobu Obara, Ph.D. [#da05421f]

Professor, Laboratory of Molecular and Cellular Physiology

Hiroshima University

Kagamiyama 1-3-1, Higashihiroshima 739-8546, Hiroshima, Japan

E-mail: &ref(e-mail-obara.jpg);

 

Ph.D. degree from Kyushu University in 1984, Research Associate in University of Occupational and Environmental Health (1979-1985), Visiting Fellow in National Cancer Institute (LMB/DCBD, Dr. KM Yamada, 1985-1988), Assistant Professor in The Institute for Medical Science, Tokyo University (1988-1991), Special Basic Science Researcher, RIKEN (1991-1993), Research Scientist, ERATO, JST (1993-1994), Associate Professor (1994-2011), and Professor in Hiroshima University (2011-Present).

 

***Research Interest: [#kc05d1f9]

1) Fibronectin and Integrin interaction in development, wound healing, and tumor metastasis

2) Physiological role of oxygen in brain during normal development and diseases

 

 

***Publications: [#na6b9480]

1) Obara M., Sakuma, T., Fujikawa, K. The third type III module of human fibronectin mediates cell adhesion and migration. Journal of Biochemistry,47, 327-335, 2010　（Impact factor(IF):1.945, Citation frequency (CF):3）

2) Adachi, T., Wang, X., Murata, T., Obara, M. Akutsu, H., Machida, M., Umezawa, A.,Tomita, M. Production of a non-helical collagen a chain in transgenic silkworms and its evaluation as a gelatin substitute for cell culture., Biotechnology and Bioengineering, 106,  860-870, 2010 (IF:3.377, CF:4)

3) Chen W, Obara M, Ishida Y, Suzuki K, Yoshizato K. Characterization of histone lysine-specific demethylase in relation to thyroid hormone-regulated anuran metamorphosis.Development Growth and Differrentiation. 49(4) :325-334,　2007　(IF:2.28, CF:0)

4) Xi Y, Obara M, Ikeda S, Yoshizato K. Gene expression and tissue distribution of cytoglobin and myoglobin in the Amphibian and Reptilia　Gene. 2007, 15; 398, 94-102、2007(IF:2.416, CF:9)

5) Ogawa T, Tateno C, Asahina K, Fujii H, Kawada N, Obara M, Yoshizato K. Identification of vitamin A-free cells in a stellate cell enriched fraction of normal rat liver as myofibroblasts　Histochemistry Cell Biology, 127,161-174, 2007　(IF;3.021, CF;10)

6) Y Tateaki, T Ogawa, N Kawada, T Kohashi, K Arihiro, C Tateno, M Obara, K Yoshizato. Typing of hepatic nonparenchymal cells using fibulin-2, and cytoglobin/STAP as liver fibrogenesis-related markers. Histochemistry and Cell Biology, 122, 41-49, 2004 (IF:3.021, CF:26)

7) Y Ishida, K Suzuki, R Utoh, M Obara, K Yoshizato. Molecular identification of the skin tranformation center of anuran larval skin using genes of rana ketatin and secreted protein acidic and rich in cysteine as probes.Development, Growth and Differentiation, 45, 515-526, 2003 (IF:2.28, CF:9)

8) K Oofusa, O Tooi, A kashiwagi, K Kashiwagi, Y Kondo, M Obara, K Yoshizato. Metal ion-responsive transgenic Xenopus laevis as an environmental monitoring animal. Environmental Toxicology and Pharmacology, 13, 153-159, 2003 (IF:1.293, CF:3)

9) T. Kato, K Miyazaki, K Nishikawa, K Koshiba, M Obara, K Yoshizato. Unique expression patterns of matrix metalloproteinases in regenerating newt limbs. Developmental Dynamics, 226, 336-376. 2002(IF3.083, CF:23)

10) Y Watanabe, R Tanaka, H kobayashi, R Utoh, K Suzuki, M Obara, K Yoshizato. Metamorphosis dependent transcriptional regulation of xak-c, a novel Xenopus type I keratin gene. Developmental Dynamics, 225, 561-570. 2002(IF:3.083, CF:16)

11) K Asahina, N Kawada, DR Kristensen, K Nakatani, S Seki, M Shiokawa, C Tateno, M Obara, K Yoshizato. Characterization of human stellate cell activation-associated protein (STAP) and its   expression   in human liver Biochmica Biophysica Acta, 1577, 471-475. 2002(IF:4.374, CF:21)

12) Suzuki, R Utoh, K Kotani, M Obara, K Yoshizato. Lineage of anuran epidermal basal cells   and their differentiation potential in relation to metamorphic skin remodeling.  Development, Growth and Differentiation, 44, 225-238. 2002 (IF:2.28, CF:20)

13) T Kohashi, U Tateaki, C Tateno, T Asahara, M Obara, K Yoshizato. Expression of pleiotropin in hepatic nonparenchymal cells and preneoplastic nodules in carbon tetrachloride-induced fibrotic rat liver.Growth factors, 20, 53-60. 2002 (IF:2.468, CF:7)

14) Nakano, T Hara, T Hayama, M Obara, K Yoshizato, K Oohama. Membrane type I matrix metalloproteinase is induced in decudual stroma without direct invasion by tropoblasts.Molecular Human Reproduction, 7, 271-277. 2001 (IF:3.005, CF:7)

15) R Utoh, K Asahina, K Suzuki, K Kotani, M Obara, K Yoshizato. Developmentally and regionally regulated participation of epidermal cells in the formation of collagne lamella of anuran tadpole skin.Development, Growth and Differentiation, 42, 571-580, 2000 (IF:2.28, CF:14)

16) Oomizu, F Sahuc, M Inamatsu, T Matsuzaki, K Asahina, M Sasaki, M Obara, K Yoshizato. Kdaf, a novel gene associated with the strafication of the epithelium.  Gene, 256, 19-27, 2000 (IF:2.416, CF:18)

17) K.Asahina, M. Obara, K.Yoshizato. Expression of genes of type I and II collagen in the formation and development of blastema of regenerating newt limb. Developmental Dynamics, 216, 59-71. 1999 (IF:3.083, CF:8)

18) K.Asahina, E Utoh, M. Obara, K. Yoshizato. Cell-type specific and thyroid hormone-dependent expression of genes of a1(I) and a2(I) collagen  in intestine during amphibian metamorphosis. Matrix Biology, 18, 89-103.1999 (IF:3.558, CF:14)

19) Y. Yokasaki, N. Matsuura, S. Higashiyama, I.  Murakami, M. Obara, M.  Yamakido, N. Shigeto, J, C.D. Sheppard. Identification of the ligand binding site for the integrin a9b1 in the third fibronectin type   III repeat of tenascin-C.Journal of Biological Chemistry, 273, 11423-11428.1999 (IF:5.328, CF:89)

20) K Yoshizato, M Tsukahara, T Oki, M Obara. The interaction of cellular fibronectin with collagen during fibroblast-mediated  contraction  of collagen gels. Journal of Investigative Dermatology Symposium Proceedings, 4, 190-195, 1999

21)  M　Obara,  K　Yoshizato. Vector-swapping strategy with an exonuclease in cloning and high expression of small PCR products. Bio-Techniques, 24, 44-46.1998 (IF:2.548, CF:0)

22) K. Asahina, K. Oofusa, M. Obara, K. Yoshizato. Cloning and characterization of the full-length cDNA encoding a2 type I collagen of bullfrog Rana catesbeiana. Gene, 194, 283-289. 1997(IF:2.416, CF:17)

23) M. Obara, K. Yoshizato. A novel domain of fibronectin revealed by epitope mapping of a monoclonal antibody which inhibits fibroblasts-mediated collagen gel contraction. FEBS Letters, 412, 48-52. 1997 (IF:3.541, CF:12)

24) M. Mukai, M. Obara, K. Yoshizato. Characterization of gene of anuran cathepsin D  as metamorphosis-regulating enzyme. Development, Growth and Differentiation, 37,463-478, 1995 (IF:2.28, CF:10)

25) M. Obara, K. Yoshizato. Possible Involvement of the a5 subunit of a5b1 integrin with the synergistic region of the central cell-binding domain of fibronectin in cells to fibronectin binding. Experimental Cell Research, 216, 273-276. 1995 (IF:3.589, CF:13)

26) H. Kawakatsu, R.  Shiurba, M. Obara, H. Hiraiwa, K. Kusakabe, T. Sakakura. Human carcinoma cell synthesize and secrete tenascin in vitro.   Japan Journal of Cancer Research (currently Cancer Science), 83,1073-1080.　1995　(IF:3.771, CF:47)

27) RD. Bouditch, CE. Halloran, S. Aota, M. Obara, EF. Plow, KM. Yamada, MH. Ginsberg. Integrin   aIIbb3　(platelet GPIIb-IIIa) recognizes multiple site in fibronectin. Journal of Biological Chemistry, 266, 23323 23328. 1991 (IF:5.328, CF:95)

28) M. Yamagata,　KM. Yamada, S.S. Yamada, Shinomura, H. Tanaka, N. Nishida, M. Obara, K. Kimata. The complete primary structures of the typeXII collagen show a chimeric molecule with reiterated fibronectin type III motif, von Willbrand factor A motifs, a domain homologous to a noncollagenous region of type IX collagen, and short collagenous domain with an Arg-Gly Asp site. Journal of Cell Biology, 115, 209-221. 1991 (IF:9.575, CF:96)

29) M. Ichinose, K. Miki, M. Tatematsu, C. Furihata, M. Matsushima, Y. Ichinose, M. Tanji, T. Konishi, M. Obara, H. Inose, K. Kuroiwa, T.Takahashi, T. Kageyama, Y. Takahashi. Hydrocortisone-induced enhancement of expression and changes in methylation of pepsinogen gene in stomach mucosa of the developing rat. Biochemical and Biophysical Research Communications, 172, 1086-1093.1990(IF:2.548, CF:15)

30) M.J. Humphries, M. Obara, K. Olden, KM. Yamada. Role of fibronectin in adhesion, migration and metastasis. Cancer Investigation, 7, 373-393. 1989 (IF:2.105, CF:72)

31) Nagata, K. Hirayashi,  A.Nakai, S. Saga, M. Obara, K.M. Yamada. A novel transformation sensitive heat shock protein (HSP 47). Hyperthermic Oncology, 2, 252-255. 1988

32) S.Dufour, J-L. Duband, M.J. Humphries, M. Obara, K.M. Yamada, J.P. Thiery. Attachment, spreading, and locomotion of avian neural crest cells are mediated by multiple  adhesion site on fibronectin molecules. EMBO Journal, 4,2661 2671. 1988 (IF:8.993, CF:178)

33) K. Nagata, K. Hirayashi, M. Obara, S. Saga, K.M. Yamada. Biosynthesis of a novel transformation-sensitive heat shock protein that binds to collagen. Journal of Biological Chemistry, 263, 8344-8349.1988 (IF:5.328, CF:49)

34) M. Obara, M.S. Kang, K.M. Yamada. Site-directed mutagenesis of the cell binding domain of human fibronectin: Separable, synergistic sites mediate adhesive function.  Cell, 53, 649-657.1988 (IF:31.152, CF:327)

35) C. Furihata, M. Obara, T. Matsushima.  In vivo short-term assay for identification of organ specific carcinogen and tumor-promoters. Journal of University of Occupational and Environmental Health, 11, 641-651.1988

36) S. Kubomura, M. Obara, Y. Karasaki,H. Taniguchi, S. Gotoh, T. Tsuda, K. Higashi, K.Osato, H.Hirano. Genetic analysis of the cell-binding domain region of the chicken fibronectin gene. Biochimica Biophysica Acta , 910,171-181. 1987 (IF:4.374, CF:11)

37) M. Obara, MS.  Kang, S. Dufour, A. Kornblihitt, J.P. Thiery, KM. Yamada. Expression of the cell binding domain of fibronectin in E.coli :Identification of sequences promoting full to minimal adhesive  function. FEBS letters, 213, 261-264. 1987 (IF:3.541, CF:35)

38) T. Tsuda, M. Obara, H. Hirano, S. Gotoh, S. Kubomura, K. Higashi, A. Kuroiwa, A. Nakagawara, K. Shimizu, Analysis of N-myc amplification in relation to disease stage and histologic type in neuroblastoma.Cancer, 60, 820-826.1987 (IF:5.418, CF:105)

39) K.Ikeuchi, M. Obara, H. Hirano, S. Gotoh,Y. Karasaki, K. Higashi. Heme metabolism and turnover of cytochrome P 450 in tumor-bearing mouse livers.Journal of University of Occupational and Environmental Health, 7,269-277.1985

40) K. Higashi, K. Ikeuchi, M.  Obara, Y. Karasaki, H. Hirano, S. Gotoh, Y. Koga, Purification of a single major form of microsomal cytochrome P-450 from tulip bulbs. Agricultural and Biological Chemistry (currently Bioscience, Biotechnology and Biochemistry) 49, 2399- 2405. 1985 (IF:1.326, CF:37)

41) M Obara. Studies on the ribosomal RNA methylation in the nucleoli from Ehrlich ascites tumor cells.Thesis, Graduate School of Science, Kyushu University, Nov. 29, 1984

42) K. Higashi, K. Ikeuchi, Y. Karasaki, M. Obara, Isolation of immunologically distinct form of cytochrome P-450 from microsome of tulip bulbs. Biochemical and Biophysical Research Communications, 115, 46-52.1983 (IF:2.548, CF:29)

43) M. Obara, K. Higashi, Recognition of the ribosomal RNA structures by purified  nucleolar methyltransferase. Journal of University of Occupational and Environmental Health, 5, 337-342,1983

44) M. Obara, H. Hirano, K. Higashi, Purification and characterization of nucleolar ribonucleic acid methylase from Ehrlich  ascites tumor cells of mice. Biochemistry, 21, 1374-1380. 1982 (IF:3.226, CF:5)

45) M. Obara, Y. Kuchino, K. Higashi, Isolation of nucleolar methylase producing only 5-methyl-cytidine in ribosomal RNA.  Biochemical and Biophysical Research Communications, 104, 241-246, 1982 (2.749) (IF:2.548, CF:5)

46) M. Obara, Y. Karasaki, M. Ohno, a-Chymotrypsin catalyzed hydrolysis of peptide substrates. Effect on the reactivity of the secondary interaction due to the peptide moiety C-terminal to the cleaved bond. Journal of Biochemistry, 86, 461-468, 1979 (IF:1.945, CF:7)